Detection of post-transcriptional RNA editing events

Methods Mol Biol. 2015:1269:189-205. doi: 10.1007/978-1-4939-2291-8_12.

Abstract

The advent of deep sequencing technologies has greatly improved the study of complex eukaryotic genomes and transcriptomes, providing the unique opportunity to investigate posttranscriptional molecular mechanisms as alternative splicing and RNA editing at single base-pair resolution. RNA editing by adenosine deamination (A-to-I) is widespread in humans and can lead to a variety of biological effects depending on the RNA type or the RNA region involved in the editing modification. Hereafter, we describe an easy and reproducible computational protocol for the identification of candidate RNA editing sites in human using deep transcriptome (RNA-Seq) and genome (DNA-Seq) sequencing data.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / genetics
  • Computational Biology / methods
  • High-Throughput Nucleotide Sequencing
  • RNA Editing / genetics*
  • Sequence Analysis, RNA

Substances

  • Adenosine