Weak D Type 4.2.2 (DAR1.2) in an African child: Serology and molecular characterization

Transfus Apher Sci. 2015 Apr;52(2):217-9. doi: 10.1016/j.transci.2014.12.020. Epub 2014 Dec 19.

Abstract

The weak D phenotype is represented by a group of RHD genotypes that code for alterated RhD proteins associated with a reduced RhD expression on red blood cell. By routine serology, some partial D variants are likely to be missed. In this report we describe the case of a three-year-old Black African child with a "unclear" reaction with monoclonal anti-D. We analyzed the blood sample of the child with different methods to conclude that it is a case of DAR 1.2 (weak D 4.2.2) and that it must be transfused with D negative erithrocytes.

Keywords: BeadChip; D-Partial; D-Weak.

Publication types

  • Case Reports

MeSH terms

  • Africa
  • Alleles
  • Black People
  • Blood Grouping and Crossmatching / methods*
  • Child, Preschool
  • DNA Mutational Analysis
  • Erythrocytes / cytology
  • Exons
  • Genetic Variation
  • Genotype
  • Haplotypes
  • Humans
  • Mutation
  • Phenotype
  • Polymerase Chain Reaction
  • Rh-Hr Blood-Group System / genetics*
  • Rho(D) Immune Globulin / blood*
  • Serology / methods*

Substances

  • RHO(D) antibody
  • Rh-Hr Blood-Group System
  • Rho(D) Immune Globulin