SIRT1 deficiency in microglia contributes to cognitive decline in aging and neurodegeneration via epigenetic regulation of IL-1β

J Neurosci. 2015 Jan 14;35(2):807-18. doi: 10.1523/JNEUROSCI.2939-14.2015.

Abstract

Aging is the predominant risk factor for neurodegenerative diseases. One key phenotype as the brain ages is an aberrant innate immune response characterized by proinflammation. However, the molecular mechanisms underlying aging-associated proinflammation are poorly defined. Whether chronic inflammation plays a causal role in cognitive decline in aging and neurodegeneration has not been established. Here we report a mechanistic link between chronic inflammation and aging microglia and a causal role of aging microglia in neurodegenerative cognitive deficits. We showed that SIRT1 is reduced with the aging of microglia and that microglial SIRT1 deficiency has a causative role in aging- or tau-mediated memory deficits via IL-1β upregulation in mice. Interestingly, the selective activation of IL-1β transcription by SIRT1 deficiency is likely mediated through hypomethylating the specific CpG sites on IL-1β proximal promoter. In humans, hypomethylation of IL-1β is strongly associated with chronological age and with elevated IL-1β transcription. Our findings reveal a novel epigenetic mechanism in aging microglia that contributes to cognitive deficits in aging and neurodegenerative diseases.

Keywords: NF-κ B; epigenetic; innate immunity; interleukin; memory deficits; neuroinflammation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / metabolism*
  • Animals
  • Case-Control Studies
  • Cognition*
  • DNA Methylation
  • Epigenesis, Genetic*
  • Humans
  • Interleukin-1beta / genetics
  • Interleukin-1beta / metabolism*
  • Mice
  • Microglia / metabolism*
  • Sirtuin 1 / deficiency
  • Sirtuin 1 / genetics
  • Sirtuin 1 / metabolism*
  • Tauopathies / metabolism
  • Up-Regulation

Substances

  • Interleukin-1beta
  • Sirt1 protein, mouse
  • Sirtuin 1