A t(3;9)(q25.1;q34.3) translocation leading to OLFM1 fusion transcripts in Gilles de la Tourette syndrome, OCD and ADHD

Psychiatry Res. 2015 Feb 28;225(3):268-75. doi: 10.1016/j.psychres.2014.12.028. Epub 2014 Dec 30.

Abstract

Gilles de la Tourette syndrome (GTS) is a neuropsychiatric disorder with a strong genetic etiology; however, finding of candidate genes is hampered by its genetic heterogeneity and the influence of non-genetic factors on disease pathogenesis. We report a case of a male patient with GTS, obsessive compulsive disorder, attention-deficit/hyperactivity-disorder, as well as other comorbidities, and a translocation t(3;9)(q25.1;q34.3) inherited from a mother with tics. Mate-pair sequencing revealed that the translocation breakpoints truncated the olfactomedin 1 (OLFM1) gene and two uncharacterized transcripts. Reverse-transcription PCR identified several fusion transcripts in the carriers, and OLFM1 expression was found to be high in GTS-related human brain regions. As OLFM1 plays a role in neuronal development it is a likely candidate gene for neuropsychiatric disorders and haploinsufficiency of OLFM1 could be a contributing risk factor to the phenotype of the carriers. In addition, one of the fusion transcripts may exert a dominant-negative or gain-of-function effect. OLFM1 is unlikely to be a major GTS susceptibility gene as no point mutations or copy number variants affecting OLFM1 were identified in 175 additional patients. The translocation described is thus a unique event, but further studies in larger cohorts are required to elucidate involvement of OLFM1 in GTS pathogenesis.

Keywords: Fusion transcripts; Microscopically balanced translocation; Neuropsychiatric disorder.; OLFM1; Tourette syndrome.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Attention Deficit Disorder with Hyperactivity / epidemiology
  • Attention Deficit Disorder with Hyperactivity / genetics*
  • Chromosomes, Human, Pair 3 / genetics*
  • Chromosomes, Human, Pair 9 / genetics*
  • Cohort Studies
  • Comorbidity
  • Denmark
  • Extracellular Matrix Proteins / genetics*
  • Glycoproteins / genetics*
  • Humans
  • Male
  • Obsessive-Compulsive Disorder / epidemiology
  • Obsessive-Compulsive Disorder / genetics*
  • Point Mutation
  • Tourette Syndrome / epidemiology
  • Tourette Syndrome / genetics*
  • Translocation, Genetic / genetics*
  • Young Adult

Substances

  • Extracellular Matrix Proteins
  • Glycoproteins
  • olfactomedin