Cationic fluorescent polymer core-shell nanoparticles for encapsulation, delivery, and non-invasively tracking the intracellular release of siRNA

Chem Commun (Camb). 2015 Feb 18;51(14):2976-9. doi: 10.1039/c4cc09685a.

Abstract

A multifunctional nanocarrier for encapsulation and delivery of short interfering RNA (siRNA) has been realized using cationic fluorescent polymer core-shell nanoparticles. The nanocarrier has good biocompatibility and high transfection efficiency over the most popular transfection reagent, Lipofectamine 2000. Fluorescence resonance energy transfer within the nanocarrier provides a non-invasive and label-free method to track the intracellular release of siRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsules
  • Drug Carriers / chemistry*
  • Drug Design
  • Drug Liberation
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Hep G2 Cells
  • Humans
  • Intracellular Space / metabolism*
  • Nanoparticles / chemistry*
  • Polymers / chemistry*
  • RNA, Small Interfering / chemistry*
  • RNA, Small Interfering / metabolism*

Substances

  • Capsules
  • Drug Carriers
  • Fluorescent Dyes
  • Polymers
  • RNA, Small Interfering