Abstract
A tumorigenicity assay was performed using DNA extracted from the human mammary carcinoma cell line MCF-7. Seven nude mouse tumors were obtained and were analyzed for the presence of human sequences and known activated oncogenes. The c-erbB.2/neu gene was identified in two tumors and two ras oncogenes in two other tumors. In order to characterize the mechanism of activation of the c-erbB.2/neu oncogene, we isolated and analyzed this gene from one of the tumors. The cloned gene did not present any rearrangement. c-erbB.2/neu transcripts from the nude mice tumors were of normal size. In one case overexpression was observed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Southern
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Breast Neoplasms / genetics*
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Breast Neoplasms / pathology
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Cell Line
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Cloning, Molecular
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Cosmids
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DNA, Neoplasm / genetics
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DNA, Neoplasm / isolation & purification
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Female
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Humans
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Mice
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Mice, Nude
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Neoplasm Transplantation
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Nucleic Acid Hybridization
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Protein-Tyrosine Kinases / genetics
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Proto-Oncogene Proteins / genetics
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Proto-Oncogenes*
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Receptors, Thyroid Hormone
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Restriction Mapping
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Transplantation, Heterologous
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Tumor Cells, Cultured / cytology*
Substances
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DNA, Neoplasm
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Proto-Oncogene Proteins
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Receptors, Thyroid Hormone
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Protein-Tyrosine Kinases