Identification of a novel human rhinovirus C type by antibody capture VIDISCA-454

Seyed Mohammad Jazaeri Farsani; Bas B Oude Munnink; Marta Canuti; Martin Deijs; Matthew Cotten; Maarten F Jebbink; Joost Verhoeven; Paul Kellam; Katherine Loens; Herman Goossens; Margareta Ieven; Lia van der Hoek

doi:10.3390/v7010239

Identification of a novel human rhinovirus C type by antibody capture VIDISCA-454

Viruses. 2015 Jan 19;7(1):239-51. doi: 10.3390/v7010239.

Authors

Affiliations

¹ Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. [email protected].
² Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. [email protected].
³ Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. [email protected].
⁴ Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. [email protected].
⁵ Department of Virus Genomics, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK. [email protected].
⁶ Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. [email protected].
⁷ Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. [email protected].
⁸ Department of Virus Genomics, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK. [email protected].
⁹ Department of Medical Microbiology, Vaccine and Infectious Disease Institute, University Hospital, Antwerp B-2650, Belgium. [email protected].
¹⁰ Department of Medical Microbiology, Vaccine and Infectious Disease Institute, University Hospital, Antwerp B-2650, Belgium. [email protected].
¹¹ Department of Medical Microbiology, Vaccine and Infectious Disease Institute, University Hospital, Antwerp B-2650, Belgium. [email protected].
¹² Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. [email protected].

Abstract

Causative agents for more than 30 percent of respiratory infections remain unidentified, suggesting that unknown respiratory pathogens might be involved. In this study, antibody capture VIDISCA-454 (virus discovery cDNA-AFLP combined with Roche 454 high-throughput sequencing) resulted in the discovery of a novel type of rhinovirus C (RV-C). The virus has an RNA genome of at least 7054 nt and carries the characteristics of rhinovirus C species. The gene encoding viral protein 1, which is used for typing, has only 81% nucleotide sequence identity with the closest known RV-C type, and, therefore, the virus represents the first member of a novel type, named RV-C54.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antibodies, Viral*
Cluster Analysis
Female
Genome, Viral*
Humans
Molecular Sequence Data
Phylogeny
Picornaviridae Infections / virology
RNA, Viral / genetics*
Respiratory System / virology
Respiratory Tract Infections / virology
Rhinovirus / classification*
Rhinovirus / immunology
Rhinovirus / isolation & purification*
Sequence Analysis, DNA*
Sequence Homology

Substances

Antibodies, Viral
RNA, Viral

Associated data

GENBANK/KP282614