Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival

Dmitriy Sergeevich Kobyakov; Ashot Merudzhanovich Avdalyan; Aleksandr Fedorovich Lazarev; Elena Leonidovna Lushnikova; Lev Moiseevich Nepomnyashchikh

doi:10.7497/j.issn.2095-3941.2014.04.005

Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival

Cancer Biol Med. 2014 Dec;11(4):264-9. doi: 10.7497/j.issn.2095-3941.2014.04.005.

Authors

Dmitriy Sergeevich Kobyakov¹, Ashot Merudzhanovich Avdalyan¹, Aleksandr Fedorovich Lazarev¹, Elena Leonidovna Lushnikova¹, Lev Moiseevich Nepomnyashchikh¹

Affiliation

¹ 1 Budget Institution, Kogalym City Hospital, Kogalym 628484, Russia ; 2 Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N. Blokhin Cancer Research Center, Barnaul 656049, Russia ; 3 Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630117, Russia.

PMID: 25610712
PMCID: PMC4296087
DOI: 10.7497/j.issn.2095-3941.2014.04.005

Abstract

Objective: To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC).

Methods: A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 (clone MIB-1) and silver nitrate by immunohistochemical and AgNOR-staining methods.

Results: The AgNOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM (tumor, node, and metastasis) system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological (AgNOR area in MIB-1-positive cells), and clinicopathological (greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation) parameters are independent prognostic factors of NSCLC.

Conclusion: The AgNOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC.

Keywords: Argyrophilic nucleolar organizer region (AgNOR); MIB-1; non-small cell lung cancer (NSCLC); survival.