Inhibition of auxin signaling in Frankia species-infected cells in Casuarina glauca nodules leads to increased nodulation

Antony Champion; Mikael Lucas; Alexandre Tromas; Virginie Vaissayre; Amandine Crabos; Issa Diédhiou; Hermann Prodjinoto; Daniel Moukouanga; Elodie Pirolles; Maïmouna Cissoko; Jocelyne Bonneau; Hassen Gherbi; Claudine Franche; Valérie Hocher; Sergio Svistoonoff; Laurent Laplaze

doi:10.1104/pp.114.255307

Inhibition of auxin signaling in Frankia species-infected cells in Casuarina glauca nodules leads to increased nodulation

Plant Physiol. 2015 Mar;167(3):1149-57. doi: 10.1104/pp.114.255307. Epub 2015 Jan 27.

Authors

Antony Champion¹, Mikael Lucas¹, Alexandre Tromas¹, Virginie Vaissayre¹, Amandine Crabos¹, Issa Diédhiou¹, Hermann Prodjinoto¹, Daniel Moukouanga¹, Elodie Pirolles¹, Maïmouna Cissoko¹, Jocelyne Bonneau¹, Hassen Gherbi¹, Claudine Franche¹, Valérie Hocher¹, Sergio Svistoonoff¹, Laurent Laplaze²

Affiliations

¹ Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des Plantes, Université Montpellier 2, F-34394 Montpellier cedex 5, France (A.C., M.L., A.T., V.V., A.C., I.D., H.P., D.M., E.P., J.B., H.G., C.F., V.H., S.S., L.L.); andLaboratoire Mixte International Adaptation des Plantes et Microorganismes Associés aux Stress Environnementaux (A.C., M.L., A.T., A.C., I.D., H.P., D.M., E.P., M.C., J.B., H.G., C.F., V.H., S.S., L.L.) and Laboratoire Commun de Microbiologie Institut de Recherche pour le Développement/Institut Sénégalais des Recherches Agricoles/Université Cheikh Anta Diop (A.C., A.T., A.C., I.D., H.P., M.C., S.S., L.L.), Centre de Recherche de Bel Air, CP 18524 Dakar, Senegal.
² Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des Plantes, Université Montpellier 2, F-34394 Montpellier cedex 5, France (A.C., M.L., A.T., V.V., A.C., I.D., H.P., D.M., E.P., J.B., H.G., C.F., V.H., S.S., L.L.); andLaboratoire Mixte International Adaptation des Plantes et Microorganismes Associés aux Stress Environnementaux (A.C., M.L., A.T., A.C., I.D., H.P., D.M., E.P., M.C., J.B., H.G., C.F., V.H., S.S., L.L.) and Laboratoire Commun de Microbiologie Institut de Recherche pour le Développement/Institut Sénégalais des Recherches Agricoles/Université Cheikh Anta Diop (A.C., A.T., A.C., I.D., H.P., M.C., S.S., L.L.), Centre de Recherche de Bel Air, CP 18524 Dakar, Senegal [email protected].

Abstract

Actinorhizal symbioses are mutualistic interactions between plants and the soil bacteria Frankia spp. that lead to the formation of nitrogen-fixing root nodules. The plant hormone auxin has been suggested to play a role in the mechanisms that control the establishment of this symbiosis in the actinorhizal tree Casuarina glauca. Here, we analyzed the role of auxin signaling in Frankia spp.-infected cells. Using a dominant-negative version of an endogenous auxin-signaling regulator, INDOLE-3-ACETIC ACID7, we established that inhibition of auxin signaling in these cells led to increased nodulation and, as a consequence, to higher nitrogen fixation per plant even if nitrogen fixation per nodule mass was similar to that in the wild type. Our results suggest that auxin signaling in Frankia spp.-infected cells is involved in the long-distance regulation of nodulation in actinorhizal symbioses.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Cell Size
Fabaceae / cytology*
Fabaceae / genetics
Fabaceae / microbiology*
Frankia / physiology*
Gene Expression Regulation, Plant
Genes, Plant
Indoleacetic Acids / metabolism*
Molecular Sequence Data
Nitrogen Fixation / genetics
Plant Proteins / chemistry
Plant Proteins / metabolism
Plant Root Nodulation* / genetics
Root Nodules, Plant / metabolism
Root Nodules, Plant / microbiology*
Signal Transduction*
Species Specificity

Substances

Indoleacetic Acids
Plant Proteins

Associated data

GENBANK/FQ315497
GENBANK/FQ316457
GENBANK/FQ316474
GENBANK/FQ317736
GENBANK/FQ319075
GENBANK/FQ321787
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GENBANK/FQ376114