Glomerular Density in Biopsy-Proven Hypertensive Nephrosclerosis

Am J Hypertens. 2015 Sep;28(9):1164-71. doi: 10.1093/ajh/hpu267. Epub 2015 Jan 27.

Abstract

Background: Previous autopsy studies suggested that a reduced nephron number is associated with increased risk of hypertension and chronic kidney disease. However, the significance of the nephron number estimated from a renal biopsy in patients with hypertensive nephrosclerosis (HNS) has not yet been elucidated.

Methods: In this cross-sectional study, we examined the clinicopathological findings of biopsy-proven HNS patients with preserved renal function (estimated glomerular filtration rate ≥ 60 ml/min/1.73 m(2)). The glomerular density (GD; the number of glomeruli per total renal cortical area) in biopsy specimens was evaluated as a surrogate of the nephron number. Renal biopsies from kidney transplant donors were used as healthy controls.

Results: A total of 58 HNS patients were enrolled. The GD value in the HNS patients was low compared with those in the kidney transplant donors (2.0 vs. 3.2 /mm(2)). These differences remained significant when globally sclerotic glomeruli were included in the calculation of the GD. Of note, the GD in HNS patients with overt proteinuria (≥1 g/day) was significantly lower than that of HNS patients with mild proteinuria (<1g/day; 1.8 vs. 2.2/mm(2), P = 0.014). In contrast, other histopathological parameters, including the severity of global glomerulosclerosis, interstitial fibrosis/tubular atrophy and arterial and arteriole lesions were comparable between the 2 HNS subgroups. In addition, the GD was identified as a factor that was associated with the amount of urinary protein excretion at biopsy, independent of other clinicopathological factors.

Conclusions: These results suggest that a low GD is a renal histological characteristic of HNS patients, especially those with overt proteinuria.

Keywords: blood pressure; glomerular density; glomerular hypertension; hypertension; hypertensive nephrosclerosis; nephron number; proteinuria; renal biopsy..

MeSH terms

  • Adult
  • Aged
  • Biopsy
  • Cross-Sectional Studies
  • Female
  • Glomerular Filtration Rate
  • Humans
  • Hypertension / complications*
  • Hypertension / diagnosis
  • Kidney Glomerulus / pathology*
  • Kidney Glomerulus / physiopathology
  • Linear Models
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Nephrosclerosis / etiology*
  • Nephrosclerosis / pathology*
  • Nephrosclerosis / physiopathology
  • Predictive Value of Tests
  • Proteinuria / etiology
  • Proteinuria / pathology
  • Retrospective Studies
  • Risk Factors
  • Young Adult