Structural insights into Cn-AMP1, a short disulfide-free multifunctional peptide from green coconut water

Mábio J Santana; Aline L de Oliveira; Luiz H K Queiroz Júnior; Santi M Mandal; Carolina O Matos; Renata de O Dias; Octavio L Franco; Luciano M Lião

doi:10.1016/j.febslet.2015.01.029

Structural insights into Cn-AMP1, a short disulfide-free multifunctional peptide from green coconut water

FEBS Lett. 2015 Feb 27;589(5):639-44. doi: 10.1016/j.febslet.2015.01.029. Epub 2015 Jan 29.

Authors

Mábio J Santana¹, Aline L de Oliveira², Luiz H K Queiroz Júnior¹, Santi M Mandal³, Carolina O Matos¹, Renata de O Dias⁴, Octavio L Franco⁵, Luciano M Lião⁶

Affiliations

¹ Institute of Chemistry, Federal University of Goiás, Goiânia, GO, Brazil.
² Institute of Chemistry, University of Brasília, Brasília, DF, Brazil.
³ Central Research Facility, Indian Institute of Technology Kharagpur, Kharagpur 721302, WB, India.
⁴ S-Inova, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil.
⁵ Centro de Analises Proteomicas e Bioquímicas, Pós-graduação em Ciências Genomicas e Biotecnologia, Brasília, DF, Brazil; S-Inova, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil. Electronic address: [email protected].
⁶ Institute of Chemistry, Federal University of Goiás, Goiânia, GO, Brazil. Electronic address: [email protected].

PMID: 25639464
DOI: 10.1016/j.febslet.2015.01.029

Abstract

Multifunctional and promiscuous antimicrobial peptides (AMPs) can be used as an efficient strategy to control pathogens. However, little is known about the structural properties of plant promiscuous AMPs without disulfide bonds. CD and NMR were used to elucidate the structure of the promiscuous peptide Cn-AMP1, a disulfide-free peptide isolated from green coconut water. Data here reported shows that peptide structure is transitory and could be different according to the micro-environment. In this regard, Cn-AMP1 showed a random coil in a water environment and an α-helical structure in the presence of SDS-d25 micelles. Moreover, deuterium exchange experiments showed that Gly4, Arg5 and Met9 residues are less accessible to solvent, suggesting that flexibility and cationic charges seem to be essential for Cn-AMP1 multiple activities.

Keywords: Antimicrobial; Cn-AMP1; Green coconut water; NMR structure; Promiscuous peptide; SDS micelles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Anti-Infective Agents / chemistry*
Circular Dichroism
Cocos / chemistry*
Disulfides / chemistry*
Magnetic Resonance Spectroscopy
Peptides / chemistry*

Substances

Anti-Infective Agents
Disulfides
Peptides