lspA gene of Mycobacterium tuberculosis co-transcribes with Rv1540 and induced by surface and acidic stress

Rakesh Pathak; Nisha Rathor; Kushal Garima; Naresh Kumar Sharma; Pooja Singh; Mandira Varma-Basil; Mridula Bose

doi:10.1016/j.gene.2015.01.061

lspA gene of Mycobacterium tuberculosis co-transcribes with Rv1540 and induced by surface and acidic stress

Gene. 2015 Apr 10;560(1):57-62. doi: 10.1016/j.gene.2015.01.061. Epub 2015 Jan 30.

Authors

Rakesh Pathak¹, Nisha Rathor², Kushal Garima³, Naresh Kumar Sharma⁴, Pooja Singh⁵, Mandira Varma-Basil⁶, Mridula Bose⁷

Affiliations

¹ Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: [email protected].
² Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: [email protected].
³ Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: [email protected].
⁴ Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: [email protected].
⁵ Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: [email protected].
⁶ Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: [email protected].
⁷ Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India. Electronic address: [email protected].

PMID: 25644771
DOI: 10.1016/j.gene.2015.01.061

Abstract

Lipoprotein signal peptidase, lspA (Rv1539), is the only known gene in mycobacterial genome for cleaving the signal sequence from prolipoprotein to form mature lipoprotein. It has been implicated in maintaining the virulence of Mycobacterium tuberculosis. The regulation of lspA had not been studied so far. Here, we identify a novel operon lspA-Rv1540 in M. tuberculosis. We detected co-transcription of the open reading frames of lspA-Rv1540 in in-vitro as well as in ex-vivo conditions. Analysis of the sequence upstream to lspA revealed a strong promoter activity that was shown to be induced significantly by surface stress and acidic environment.

Keywords: Mycobacterium tuberculosis; Regulation; lspA promoter.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acids / pharmacology*
Aspartic Acid Endopeptidases / genetics*
Bacterial Proteins / genetics*
Gene Expression Regulation, Bacterial / drug effects
Intramolecular Transferases / genetics*
Mycobacterium tuberculosis / genetics*
Operon / drug effects
Operon / genetics
Promoter Regions, Genetic
Stress, Mechanical
Stress, Physiological* / drug effects
Stress, Physiological* / genetics
Surface Properties
Transcription, Genetic / drug effects

Substances

Acids
Bacterial Proteins
Aspartic Acid Endopeptidases
signal peptidase II
Intramolecular Transferases
pseudouridine synthases