CD40L-adjuvanted DNA/modified vaccinia virus Ankara simian immunodeficiency virus (SIV) vaccine enhances protection against neutralization-resistant mucosal SIV infection

J Virol. 2015 Apr;89(8):4690-5. doi: 10.1128/JVI.03527-14. Epub 2015 Feb 4.

Abstract

Here, we show that a CD40L-adjuvanted DNA/modified vaccinia virus Ankara (MVA) simian immunodeficiency virus (SIV) vaccine enhances protection against a pathogenic neutralization-resistant mucosal SIV infection, improves long-term viral control, and prevents AIDS. Analyses of serum IgG antibodies to linear peptides of SIV Env revealed a strong response to V2, with targeting of fewer epitopes in the immunodominant region of gp41 (gp41-ID) and the V1 region as a correlate for enhanced protection. Greater expansion of antiviral CD8 T cells in the gut correlated with long-term viral control.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Adjuvants, Immunologic / administration & dosage
  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • CD40 Ligand / administration & dosage
  • CD40 Ligand / pharmacology
  • CD8-Positive T-Lymphocytes / immunology*
  • Epitope Mapping
  • Immunity, Cellular
  • Immunoglobulin G / blood
  • Kaplan-Meier Estimate
  • Macaca mulatta
  • SAIDS Vaccines / genetics
  • SAIDS Vaccines / immunology
  • SAIDS Vaccines / pharmacology*
  • Simian Acquired Immunodeficiency Syndrome / immunology
  • Simian Acquired Immunodeficiency Syndrome / prevention & control*
  • Vaccinia virus / genetics
  • Vaccinia virus / immunology*

Substances

  • Adjuvants, Immunologic
  • Immunoglobulin G
  • SAIDS Vaccines
  • CD40 Ligand