Evaluation of a PfHRP-2 based rapid diagnostic test versus microscopy method among HIV-positive and unknown serology patients in Ouagadougou, Burkina Faso

Am J Trop Med Hyg. 2015 Apr;92(4):834-7. doi: 10.4269/ajtmh.13-0757. Epub 2015 Feb 9.

Abstract

We evaluated the performance of a malaria rapid diagnostic test (RDT; Malaria Quick Test(®); Cypress Diagnostic) compared with the standard thick-smear microscopy method using blood samples from human immunodeficiency virus (HIV)-infected individuals and individuals of unknown HIV status collected in Ouagadougou, Burkina Faso. Our results show that 42.1% of 114 HIV-infected patients were concordantly RDT- and thick smear-positive, and 55.3% were concordantly negative. Sensitivity and specificity of the RDT test were 100.0% and 95.4%, respectively, with 5.9% false-positive results and a total agreement of 97.4%; 127 patients with unknown HIV serology were analyzed; of them, 40.9% were RDT- and thick smear-positive, and 46.4% concordantly negative. Sensitivity and specificity were 100.0% and 78.6%, respectively, with 23.5% false-positive results and a total agreement of 87.4%. Malaria Quick Test(®) is rapid and effective for the diagnosis of malaria and has a high sensitivity, confirming its use in general and HIV patients in particular.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Adult
  • Antigens, Protozoan / blood*
  • Burkina Faso
  • Child
  • Child, Preschool
  • Diagnostic Tests, Routine / methods*
  • Female
  • HIV Infections / complications*
  • HIV Seropositivity
  • Humans
  • Malaria, Falciparum / complications
  • Malaria, Falciparum / diagnosis*
  • Malaria, Falciparum / parasitology
  • Male
  • Middle Aged
  • Plasmodium falciparum / immunology*
  • Plasmodium falciparum / isolation & purification
  • Predictive Value of Tests
  • Protozoan Proteins / blood*
  • Reagent Kits, Diagnostic*
  • Sensitivity and Specificity
  • Time Factors
  • Young Adult

Substances

  • Antigens, Protozoan
  • HRP-2 antigen, Plasmodium falciparum
  • Protozoan Proteins
  • Reagent Kits, Diagnostic