Short-arm centrifugation as a partially effective musculoskeletal countermeasure during 5-day head-down tilt bed rest--results from the BRAG1 study

Eur J Appl Physiol. 2015 Jun;115(6):1233-44. doi: 10.1007/s00421-015-3120-1. Epub 2015 Feb 11.

Abstract

Purpose: Human centrifugation, also called artificial gravity (AG), is proposed as a combined strategy against detrimental effects of microgravity in long-term space missions. This study scrutinized human short-arm centrifugation as countermeasure against musculoskeletal de-conditioning.

Method: Eleven healthy male subjects [mean age of 34 (SD 7) years] completed the cross-over trial, including three campaigns of -6° head-down tilt bed rest (HDT) for 5 days, with preceding baseline data collection and recovery phases. Bed rest without AG was used as control condition (Ctrl), and AG with 1 g at the center of mass applied once per day for 30 min in one bout (AG1×30) and in 6 bouts of 5 min (AG6×5, 3-min rest between bouts) as experimental conditions. End-points were muscle strength, vertical jump performance, and biomarkers of bone and protein metabolism.

Result: AG6×5 was better tolerated than AG1×30. Bone resorption markers CTX, NTX, and DPD all increased by approximately 25 % toward the end of bed rest (P < 0.001), and nitrogen balance decreased by approximately 3 g/day (P < 0.001), without any protection by AG (P > 0.4). Decreases in vertical jump height by 2.1 (SE 0.6) cm after Ctrl bed rest was prevented by either of the AG protocols (P = 0.039).

Conclusion: The present study yielded succinct catabolic effects upon muscle and bone metabolism that were un-prevented by AG. The preservation of vertical jump performance by AG in this study is likely caused by central nervous rather than by peripheral musculoskeletal effects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acids / metabolism
  • Bed Rest*
  • Bone Resorption / etiology
  • Bone Resorption / prevention & control*
  • Bone and Bones / metabolism
  • Bone and Bones / physiology
  • Centrifugation
  • Collagen Type I / metabolism
  • Gravity, Altered*
  • Head-Down Tilt*
  • Humans
  • Male
  • Movement
  • Muscle Weakness / etiology
  • Muscle Weakness / prevention & control*
  • Muscle, Skeletal / metabolism
  • Muscle, Skeletal / physiology
  • Peptide Fragments / metabolism
  • Peptides / metabolism
  • Procollagen / metabolism

Substances

  • Amino Acids
  • Collagen Type I
  • Peptide Fragments
  • Peptides
  • Procollagen
  • collagen type I trimeric cross-linked peptide
  • procollagen Type I N-terminal peptide
  • deoxypyridinoline