Occupational exposure to diesel engine exhaust and alterations in lymphocyte subsets

Occup Environ Med. 2015 May;72(5):354-9. doi: 10.1136/oemed-2014-102556. Epub 2015 Feb 11.

Abstract

Background: The International Agency for Research on Cancer recently classified diesel engine exhaust (DEE) as a Group I carcinogen based largely on its association with lung cancer. However, the exposure-response relationship is still a subject of debate and the underlying mechanism by which DEE causes lung cancer in humans is not well understood.

Methods: We conducted a cross-sectional molecular epidemiology study in a diesel engine truck testing facility of 54 workers exposed to a wide range of DEE (ie, elemental carbon air levels, median range: 49.7, 6.1-107.7 µg/m(3)) and 55 unexposed comparable controls.

Results: The total lymphocyte count (p=0.00044) and three of the four major lymphocyte subsets (ie, CD4+ T cells (p=0.00019), CD8+ T cells (p=0.0058) and B cells (p=0.017)) were higher in exposed versus control workers and findings were highly consistent when stratified by smoking status. In addition, there was evidence of an exposure-response relationship between elemental carbon and these end points (ptrends<0.05), and CD4+ T cell levels were significantly higher in the lowest tertile of DEE exposed workers compared to controls (p=0.012).

Conclusions: Our results suggest that DEE exposure is associated with higher levels of cells that play a key role in the inflammatory process, which is increasingly being recognised as contributing to the aetiology of lung cancer.

Impact: This study provides new insights into the underlying mechanism of DEE carcinogenicity.

Keywords: Diesel; lymphocyte subset; occupational exposure; toxicity.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Adult
  • Air Pollutants, Occupational* / analysis
  • B-Lymphocytes / metabolism*
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8-Positive T-Lymphocytes / metabolism
  • Carbon / analysis
  • Carcinogens / analysis
  • Cross-Sectional Studies
  • Humans
  • Inflammation / etiology
  • Inflammation / metabolism
  • Lung Neoplasms / etiology*
  • Lung Neoplasms / metabolism
  • Lymphocyte Subsets / metabolism*
  • Middle Aged
  • Motor Vehicles
  • Occupational Exposure / adverse effects*
  • Occupational Exposure / analysis
  • Risk Assessment
  • T-Lymphocytes / metabolism*
  • Vehicle Emissions* / analysis

Substances

  • Air Pollutants, Occupational
  • Carcinogens
  • Vehicle Emissions
  • Carbon