Development of imidazoline-2-thiones based two-photon fluorescence probes for imaging hypochlorite generation in a co-culture system

Angew Chem Int Ed Engl. 2015 Apr 13;54(16):4890-4. doi: 10.1002/anie.201500537. Epub 2015 Feb 20.

Abstract

We designed and prepared the imidazoline-2-thione containing OCl(-) probes, PIS and NIS, which operate through specific reactions with OCl(-) that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl(-) generation in macrophages in a co-culture system. We have also employed two-photon microscopy and PIS to image OCl(-) in live cells and tissues, indicating that this probe could have wide biological applications.

Keywords: co-culture systems; fluorescence probes; hypochlorite generation; two-photon microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Coculture Techniques
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Hippocampus / drug effects
  • Humans
  • Hypochlorous Acid / analysis*
  • Imidazolines / chemistry
  • Interferon-gamma / pharmacology
  • Lipopolysaccharides / toxicity
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice
  • Microscopy, Fluorescence, Multiphoton
  • Photons
  • Rats
  • Reactive Oxygen Species / metabolism
  • Tetradecanoylphorbol Acetate / pharmacology
  • Thiones / chemistry*

Substances

  • Fluorescent Dyes
  • Imidazolines
  • Lipopolysaccharides
  • Reactive Oxygen Species
  • Thiones
  • Hypochlorous Acid
  • Interferon-gamma
  • Tetradecanoylphorbol Acetate