Abstract
Members of the SNARE-family of proteins are known to be key regulators of the membrane-membrane fusion events required for intracellular membrane traffic. The ubiquitously expressed SNARE protein SNAP-23 regulates a wide variety of exocytosis events and is essential for mouse development. Germline deletion of SNAP-23 results in early embryonic lethality in mice, and for this reason we now describe mice and cell lines in which SNAP-23 can be conditionally-deleted using Cre-lox technology. Deletion of SNAP-23 in CD19-Cre expressing mice prevents B lymphocyte development and deletion of SNAP-23 using a variety of T lymphocyte-specific Cre mice prevents T lymphocyte development. Acute depletion of SNAP-23 in mouse fibroblasts leads to rapid apoptotic cell death. These data highlight the importance of SNAP-23 for cell survival and describe a mouse in which specific cell types can be eliminated by expression of tissue-specific Cre-recombinase.
Publication types
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Research Support, N.I.H., Intramural
MeSH terms
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Animals
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Antigens, CD19 / metabolism
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Apoptosis / genetics
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B-Lymphocytes / cytology
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B-Lymphocytes / metabolism
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Blotting, Western
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / metabolism
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CD8-Positive T-Lymphocytes / cytology
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CD8-Positive T-Lymphocytes / metabolism
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Cell Survival / genetics
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Embryo, Mammalian / cytology
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Fibroblasts / cytology
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Fibroblasts / metabolism*
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Genes, Essential / genetics*
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / metabolism
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Mice, Knockout
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Mice, Transgenic
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Microscopy, Confocal
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Qb-SNARE Proteins / genetics
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Qb-SNARE Proteins / metabolism*
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Qc-SNARE Proteins / genetics
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Qc-SNARE Proteins / metabolism*
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Spleen / cytology
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Spleen / metabolism
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Thymocytes / cytology
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Thymocytes / metabolism
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Time-Lapse Imaging
Substances
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Antigens, CD19
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Qb-SNARE Proteins
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Qc-SNARE Proteins
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Snap23 protein, mouse
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck)