Abstract
Achyrocline alata, known as Jateí-ka-há, is traditionally used to treat several health problems, including inflammations and infections. This study aimed to optimize an active extract against Streptococcus mutans, the main bacteria that causes caries. The extract was developed using an accelerated solvent extraction and chemometric calculations. Factorial design and response surface methodologies were used to determine the most important variables, such as active compound selectivity. The standardized extraction recovered 99% of the four main compounds, gnaphaliin, helipyrone, obtusifolin and lepidissipyrone, which represent 44% of the extract. The optimized extract of A. alata has a MIC of 62.5 μg/mL against S. mutans and could be used in mouth care products.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Achyrocline / chemistry*
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Achyrocline / metabolism
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Animals
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Anti-Infective Agents / chemistry*
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Anti-Infective Agents / isolation & purification
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Anti-Infective Agents / pharmacology
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Cell Line
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Chromatography, High Pressure Liquid
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Hexanes / chemistry
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Hydrogen Peroxide / metabolism
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Liquid-Liquid Extraction
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Mice
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Microbial Sensitivity Tests
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Nitric Oxide / metabolism
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Plant Extracts / chemistry*
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Plant Leaves / chemistry
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Plant Leaves / metabolism
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Plant Stems / chemistry
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Plant Stems / metabolism
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Research Design
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Streptococcus mutans / drug effects
Substances
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Anti-Infective Agents
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Hexanes
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Plant Extracts
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Nitric Oxide
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Hydrogen Peroxide
Grants and funding
The authors express their gratitude to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT) and Instituto Nacional de Áreas Úmidas (INAU) for their support in carrying out the study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.