Abstract
Saporin, a ribosome-inactivating protein from the seeds of Saponaria officinalis, was covalently linked to an anti-CD4 monoclonal antibody. The resulting immunotoxin at 10(-9)M concentration was toxic to CD4+ lymphocytes without affecting other cells. Selective elimination of CD4+ and CD8+ cells was also obtained with murine monoclonal anti-CD4 and anti-CD8 antibodies and an immunotoxin consisting of saporin linked to an anti-mouse IgG antibody.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / pharmacology*
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CD4-Positive T-Lymphocytes / drug effects*
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Cell Survival / drug effects
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Humans
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Immunotoxins / pharmacology*
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In Vitro Techniques
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N-Glycosyl Hydrolases*
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Plant Proteins / pharmacology*
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Ribosome Inactivating Proteins, Type 1
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Saporins
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T-Lymphocytes, Regulatory / drug effects*
Substances
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Antineoplastic Agents, Phytogenic
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Immunotoxins
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Plant Proteins
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Ribosome Inactivating Proteins, Type 1
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N-Glycosyl Hydrolases
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Saporins