ENU mutagenesis in the mouse

Curr Protoc Mouse Biol. 2014 Jun 16;4(2):25-35. doi: 10.1002/9780470942390.mo140029.

Abstract

This article describes the treatment of laboratory mice with the mutagen N-ethyl-N-nitrosourea (ENU) to induce very highly increased rates of mutation throughout the genome. Further, it describes several popular mating schemes designed to produce animals displaying phenotypes associated with the induced mutations.

Keywords: alkylating agent; genome sequencing; per locus mutation frequency; phenotype-driven mutagenesis; spermatogonial stem cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Ethylnitrosourea / pharmacology*
  • Genome / drug effects*
  • Humans
  • Mice / genetics*
  • Models, Animal*
  • Mutagenesis*
  • Mutagens / pharmacology*
  • Phenotype

Substances

  • Mutagens
  • Ethylnitrosourea