Abstract
Enterotoxigenic Escherichia coli fimbriae are immunogenic and play a key role in intestinal colonization. Native colonization factor antigen I, coli surface antigen 1, and coli surface antigen 3 fimbriae were purified by a common method involving shearing, differential centrifugation, gel filtration, and density gradient ultracentrifugation. The compositions and N-terminal sequences were determined. Coli surface antigen 3 possesses two N-terminal isoforms, one of which matches the published DNA sequence, except for the previously proposed signal sequence cleavage point.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Amino Acids / analysis
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Antigens, Bacterial
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Antigens, Surface / analysis*
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Bacterial Proteins / isolation & purification
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Escherichia coli / analysis*
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Escherichia coli / pathogenicity
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Fimbriae Proteins*
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Fimbriae, Bacterial / analysis*
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Molecular Sequence Data
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Molecular Weight
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Sequence Homology, Nucleic Acid
Substances
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Amino Acids
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Antigens, Bacterial
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Antigens, Surface
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Bacterial Proteins
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colonization factor antigens
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Fimbriae Proteins