Abstract
We have used a cDNA probe for the human IgE receptor alpha chain to determine the chromosomal location for the human gene. A combination of Southern blot analysis of panels of somatic-cell hybrid DNAs and chromosomal in situ hybridization has localized the gene to the long arm of chromosome 1 at q21-q23. With this cDNA probe, we have also identified an RsaI RFLP which is inherited in a Mendelian fashion. This polymorphic marker on chromosome 1 should be valuable for studies directed at the identification both of diseases involving the IgE receptor alpha chain and of genetic factors affecting the allergic response.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Antigens, Differentiation, B-Lymphocyte / genetics*
-
Chromosome Mapping
-
Chromosomes, Human, Pair 1*
-
Cloning, Molecular
-
DNA Probes
-
Deoxyribonucleases, Type II Site-Specific
-
Humans
-
Hybrid Cells
-
Immunoglobulin E
-
Mast Cells / physiology*
-
Nucleic Acid Hybridization
-
Polymorphism, Restriction Fragment Length
-
Receptors, Fc / genetics*
-
Receptors, IgE
Substances
-
Antigens, Differentiation, B-Lymphocyte
-
DNA Probes
-
Receptors, Fc
-
Receptors, IgE
-
Immunoglobulin E
-
Deoxyribonucleases, Type II Site-Specific
-
GTAC-specific type II deoxyribonucleases