Abstract
The transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is crucial for immune responses and skeletal development. Work in recent years has shown that various members of the NF-κB family are viable targets to regulate activity and survival of bone cells and hence bone metabolism. In this regard, deletion of upstream kinases or distal NF-κB subunits resulted with bone deformities. Thus, it has become increasingly apparent that detailed investigation of NF-κB in bone cells may provide opportunities to design new therapeutic modalities. In this chapter we present modified methodology describing efficient approaches to regulate the NF-κB pathway in vitro and in vivo to assess its function in bone cells and tissues.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western / methods
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Bone Marrow Cells / cytology
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Bone Marrow Cells / metabolism
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Bone Resorption / metabolism
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Cell Differentiation / drug effects
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Electrophoretic Mobility Shift Assay / methods
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Enzyme Activation / drug effects
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I-kappa B Kinase / metabolism
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Intracellular Signaling Peptides and Proteins / metabolism
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Mice
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Myeloid Progenitor Cells / metabolism*
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NF-kappa B / metabolism*
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Osteoclasts / cytology
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Osteoclasts / drug effects
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Osteoclasts / metabolism*
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Peptides / chemical synthesis
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Peptides / isolation & purification
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Peptides / pharmacology
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Protein Binding
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Signal Transduction* / drug effects
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Sumoylation
Substances
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Intracellular Signaling Peptides and Proteins
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NBD peptide, mouse
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NEMO protein, mouse
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NF-kappa B
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Peptides
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I-kappa B Kinase