The genome of Shigella dysenteriae strain Sd1617 comparison to representative strains in evaluating pathogenesis

Ajchara A Vongsawan; Vinayak Kapatral; Benjamin Vaisvil; Henry Burd; Oralak Serichantalergs; Malabi M Venkatesan; Carl J Mason

doi:10.1093/femsle/fnv011

The genome of Shigella dysenteriae strain Sd1617 comparison to representative strains in evaluating pathogenesis

FEMS Microbiol Lett. 2015 Mar;362(5):fnv011. doi: 10.1093/femsle/fnv011. Epub 2015 Jan 28.

Authors

Ajchara A Vongsawan¹, Vinayak Kapatral², Benjamin Vaisvil², Henry Burd², Oralak Serichantalergs³, Malabi M Venkatesan⁴, Carl J Mason³

Affiliations

¹ Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand [email protected] [email protected] [email protected].
² Igenbio Inc., Chicago, IL 60607, USA.
³ Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand.
⁴ Walter Reed Army Institute of Research, Division of Bacterial and Rickettsial Diseases, Silver Spring, MD 20910, USA.

Abstract

We sequenced and analyzed Shigella dysenteriae strain Sd1617 serotype 1 that is widely used as model strain for vaccine design, trials and research. A combination of next-generation sequencing platforms and assembly yielded two contigs representing a chromosome size of 4.34 Mb and the large virulence plasmid of 177 kb. This genome sequence is compared with other Shigella genomes in order to understand gene complexity and pathogenic factors.

Keywords: Shigella dysenteriae; comparison; genome.

Publication types

Comparative Study
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Base Sequence
Chromosome Mapping
Genes, Bacterial
Genome, Bacterial*
Molecular Sequence Data
Plasmids
Shigella / genetics
Shigella dysenteriae / genetics*
Shigella dysenteriae / pathogenicity*
Virulence / genetics*

Associated data

GENBANK/CP006736
GENBANK/CP006737

Grants and funding

Y1-AI-4906-03/AI/NIAID NIH HHS/United States