False-positive Aspergillus galactomannan and its kinetics in allogeneic hematopoietic stem cell transplantation

J Infect. 2015 May;70(5):520-40. doi: 10.1016/j.jinf.2015.02.012. Epub 2015 Mar 3.

Abstract

Objectives: We evaluated the incidence of and risk factors for false-positive Aspergillus galactomannan (GM) antigenemia in allogeneic hematopoietic stem cell transplantation (HSCT). We also focused on the GM index value and its kinetics.

Methods: Patients who underwent their first allogeneic HSCT at our center between June 2007 and December 2012 were included (n = 172). Episodes of positive GM tests were classified as either "true-positive", which fulfilled the EORTC criteria for proven or probable invasive aspergillosis (IA), or "false-positive", which was not accompanied by clinical findings. The remaining cases were regarded as "inconclusive".

Results: The one-year cumulative incidences of IA and positive GM tests were 10.1% and 48.1%, respectively. Among 148 episodes of positive GM tests, 97(65.5%), 23(15.5%), and 28(19.0%) were classified as false-positive, true-positive and inconclusive, respectively. In the first episodes of positive GM tests in each patient (false-positive = 67, others = 30), an increase in the GM value in the first two measurements, neutropenia, and use of anti-mold agents at positive GM episode were associated with a significantly lower possibility of false-positive results according to a multivariate analysis.

Conclusions: A false-positive GM test was frequently seen after allogeneic HSCT. An increase in the GM value may increase its positive predictive value.

Keywords: Allogeneic hematopoietic stem cell transplantation; Aspergillus galactomannan; False-positive; Kinetics of Aspergillus galactomannan.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aspergillosis / diagnosis*
  • Aspergillosis / drug therapy
  • Aspergillus / chemistry
  • Aspergillus / isolation & purification
  • Aspergillus / pathogenicity
  • Enzyme-Linked Immunosorbent Assay
  • False Positive Reactions
  • Female
  • Galactose / analogs & derivatives
  • Hematopoietic Stem Cell Transplantation / adverse effects*
  • Humans
  • Incidence
  • Kinetics
  • Male
  • Mannans / analysis*
  • Middle Aged
  • Neutropenia / complications
  • Retrospective Studies
  • Risk Factors
  • Time Factors
  • Transplantation, Homologous / adverse effects
  • Young Adult

Substances

  • Mannans
  • galactomannan
  • Galactose