A novel technique for detecting antibiotic-resistant typhoid from rapid diagnostic tests

J Clin Microbiol. 2015 May;53(5):1758-60. doi: 10.1128/JCM.00531-15. Epub 2015 Mar 11.

Abstract

Fluoroquinolone-resistant typhoid is increasing. An antigen-detecting rapid diagnostic test (RDT) can rapidly diagnose typhoid from blood cultures. A simple, inexpensive molecular technique performed with DNA from positive RDTs accurately identified gyrA mutations consistent with phenotypic susceptibility testing results. Field diagnosis combined with centralized molecular resistance testing could improve typhoid management and surveillance in low-resource settings.

Publication types

  • Evaluation Study
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Anti-Bacterial Agents / pharmacology
  • Bacteriological Techniques / methods*
  • Child
  • Child, Preschool
  • DNA Gyrase / genetics
  • DNA, Bacterial / genetics
  • Diagnostic Tests, Routine / methods*
  • Drug Resistance, Bacterial*
  • Female
  • Fluoroquinolones / pharmacology
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Middle Aged
  • Molecular Diagnostic Techniques / methods
  • Pilot Projects
  • Polymerase Chain Reaction
  • Prospective Studies
  • Salmonella typhi / drug effects*
  • Salmonella typhi / isolation & purification*
  • Time Factors
  • Typhoid Fever / diagnosis*
  • Typhoid Fever / microbiology*
  • Young Adult

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Fluoroquinolones
  • DNA Gyrase