Evaluation of an immunochromatographic assay for detection of PBP2a on non-Staphylococcus aureus clinical isolates

Benoit Mantion; Laurent Cavalié; Marie-Françoise Prère

doi:10.1016/j.mimet.2015.02.014

Evaluation of an immunochromatographic assay for detection of PBP2a on non-Staphylococcus aureus clinical isolates

J Microbiol Methods. 2015 May:112:46-8. doi: 10.1016/j.mimet.2015.02.014. Epub 2015 Mar 11.

Authors

Benoit Mantion¹, Laurent Cavalié¹, Marie-Françoise Prère²

Affiliations

¹ Laboratoire de Bactériologie-Hygiène, Chu-Purpan, Toulouse, France.
² Laboratoire de Bactériologie-Hygiène, Chu-Purpan, Toulouse, France; Faculté de médecine, Université Paul Sabatier, Toulouse, France; CNRS UMR5100, Université Paul Sabatier, Toulouse, France. Electronic address: [email protected].

PMID: 25769529
DOI: 10.1016/j.mimet.2015.02.014

Abstract

We evaluated the immunochromatographic assay PBP2a Culture Colony Test (Alere™) on 50 samples of methicillin resistant and sensitive non-Staphylococcus aureus isolates belonging to ten species. Because it is rapid and reliable, this test should be advantageous as routine test in place of mecA/C PCR.

Keywords: Immunochromatographic assay; Methicillin; Non-Staphylococcus aureus; PBP2a; mecA/C.

Publication types

Evaluation Study

MeSH terms

Bacteria / chemistry*
Bacteria / drug effects*
Bacterial Proteins / analysis*
Chromatography, Affinity / methods*
Methicillin Resistance*

Substances

Bacterial Proteins