Genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain NZP2037

Simon Kelly; John Sullivan; Clive Ronson; Rui Tian; Lambert Bräu; Karen Davenport; Hajnalka Daligault; Tracy Erkkila; Lynne Goodwin; Wei Gu; Christine Munk; Hazuki Teshima; Yan Xu; Patrick Chain; Tanja Woyke; Konstantinos Liolios; Amrita Pati; Konstantinos Mavromatis; Victor Markowitz; Natalia Ivanova; Nikos Kyrpides; Wayne Reeve

doi:10.1186/1944-3277-9-7

Genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain NZP2037

Stand Genomic Sci. 2014 Dec 8:9:7. doi: 10.1186/1944-3277-9-7. eCollection 2014.

Authors

Simon Kelly¹, John Sullivan¹, Clive Ronson¹, Rui Tian², Lambert Bräu³, Karen Davenport⁴, Hajnalka Daligault⁴, Tracy Erkkila⁴, Lynne Goodwin⁴, Wei Gu⁴, Christine Munk⁴, Hazuki Teshima⁴, Yan Xu⁴, Patrick Chain⁴, Tanja Woyke⁵, Konstantinos Liolios⁵, Amrita Pati⁵, Konstantinos Mavromatis⁶, Victor Markowitz⁶, Natalia Ivanova⁵, Nikos Kyrpides⁷, Wayne Reeve²

Affiliations

¹ Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
² Centre for Rhizobium Studies, Murdoch University, Murdoch, Perth, Australia.
³ School of Life and Environmental Sciences, Deakin University, Deakin, Victoria, Australia.
⁴ Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM, USA.
⁵ DOE Joint Genome Institute, Walnut Creek, CA, USA.
⁶ Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
⁷ DOE Joint Genome Institute, Walnut Creek, CA, USA ; Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

Abstract

Mesorhizobium loti strain NZP2037 was isolated in 1961 in Palmerston North, New Zealand from a Lotus divaricatus root nodule. Compared to most other M. loti strains, it has a broad host range and is one of very few M. loti strains able to form effective nodules on the agriculturally important legume Lotus pedunculatus. NZP2037 is an aerobic, Gram negative, non-spore-forming rod. This report reveals that the genome of M. loti strain NZP2037 does not harbor any plasmids and contains a single scaffold of size 7,462,792 bp which encodes 7,318 protein-coding genes and 70 RNA-only encoding genes. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

Keywords: Alphaproteobacteria; Nitrogen fixation; Root-nodule bacteria; Symbiosis.