Draft Genome Sequence of Thauera sp. Strain SWB20, Isolated from a Singapore Wastewater Treatment Facility Using Gel Microdroplets

Armand E K Dichosa; Karen W Davenport; Po-E Li; Sanaa A Ahmed; Hajnalka Daligault; Cheryl D Gleasner; Yuliya Kunde; Kim McMurry; Chien-Chi Lo; Krista G Reitenga; Ashlynn R Daughton; Xiaohong Shen; Seth Frietze; Dongping Wang; Shannon L Johnson; Daniela I Drautz-Moses; Stephan Schuster; Patrick S Chain; Cliff Han

doi:10.1128/genomeA.00132-15

Draft Genome Sequence of Thauera sp. Strain SWB20, Isolated from a Singapore Wastewater Treatment Facility Using Gel Microdroplets

Genome Announc. 2015 Mar 19;3(2):e00132-15. doi: 10.1128/genomeA.00132-15.

Authors

Armand E K Dichosa¹, Karen W Davenport², Po-E Li², Sanaa A Ahmed², Hajnalka Daligault², Cheryl D Gleasner², Yuliya Kunde², Kim McMurry², Chien-Chi Lo², Krista G Reitenga², Ashlynn R Daughton², Xiaohong Shen², Seth Frietze², Dongping Wang², Shannon L Johnson², Daniela I Drautz-Moses³, Stephan Schuster³, Patrick S Chain², Cliff Han²

Affiliations

¹ Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, USA [email protected] [email protected].
² Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
³ Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore.

Abstract

We report here the genome sequence of Thauera sp. strain SWB20, isolated from a Singaporean wastewater treatment facility using gel microdroplets (GMDs) and single-cell genomics (SCG). This approach provided a single clonal microcolony that was sufficient to obtain a 4.9-Mbp genome assembly of an ecologically relevant Thauera species.