Identification and characterization of β-sitosterol target proteins

Brett Lomenick; Heping Shi; Jing Huang; Chuo Chen

doi:10.1016/j.bmcl.2015.03.007

Identification and characterization of β-sitosterol target proteins

Bioorg Med Chem Lett. 2015 Nov 1;25(21):4976-4979. doi: 10.1016/j.bmcl.2015.03.007. Epub 2015 Mar 11.

Authors

Brett Lomenick¹, Heping Shi², Jing Huang³, Chuo Chen⁴

Affiliations

¹ Department of Molecular and Medical Pharmacology, 23-231 Center for Health Sciences, 650 Charles E Young Dr. South, University of California Los Angeles, Los Angeles, CA 90095, USA. Electronic address: [email protected].
² Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.
³ Department of Molecular and Medical Pharmacology, 23-231 Center for Health Sciences, 650 Charles E Young Dr. South, University of California Los Angeles, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, CA 90095, USA. Electronic address: [email protected].
⁴ Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA. Electronic address: [email protected].

Abstract

β-Sitosterol is the most abundant plant sterol in the human diet. It is also the major component of several traditional medicines, including saw palmetto and devil's claw. Although β-sitosterol is effective against enlarged prostate in human clinical trials and has anti-cancer and anti-inflammatory activities, the mechanisms of action are poorly understood. Here, we report the identification of two new binding proteins for β-sitosterol that may underlie its beneficial effects.

Keywords: 17β-HSD4; E-Syt1; Phytosterol; Target identification; β-Sitosterol.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Binding Sites
Humans
Lipopolysaccharides / pharmacology
Macrophages / cytology
Macrophages / drug effects
Mice
Molecular Conformation
Peroxisomal Multifunctional Protein-2 / chemistry
Peroxisomal Multifunctional Protein-2 / metabolism*
Protein Binding
Sitosterols / chemistry
Sitosterols / metabolism*
Sitosterols / pharmacology*
Synaptotagmin I / chemistry
Synaptotagmin I / metabolism*

Substances

Lipopolysaccharides
Sitosterols
Synaptotagmin I
gamma-sitosterol
Peroxisomal Multifunctional Protein-2

Abstract

Publication types

MeSH terms

Substances

Grants and funding