Identification of sequence polymorphisms in the D-loop region of mitochondrial DNA as risk biomarker for liposarcoma

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3403-4. doi: 10.3109/19401736.2015.1018240. Epub 2015 Mar 26.

Abstract

Single nucleotide polymorphisms (SNPs) in the Displacement-loop (D-loop) region of mitochondrial DNA have been reported to be associated with cancer risk in various types of cancer. To assess the frequency of D-loop SNPs in a large series of liposarcoma and establish correlations with cancer risk, we sequenced the D-loop of 82 liposarcoma patients and analyzed their use as predictive biomarkers for liposarcoma risk. The minor alleles of nucleotides 73G, 523-524del, 16,290T, 16,319A, 16,356C were associated with an increased risk for liposarcoma patients, whereas the insertion of C at the site 315 (located within the D310) were associated with a decreased risk for liposarcoma patients. These results suggest that SNPs in the mitochondrial D-loop should be considered as a biomarker which may be useful for the early detection of liposarcoma in individuals at risk of this cancer.

Keywords: D-loop; SNP; liposarcoma; mtDNA.

MeSH terms

  • Alleles
  • Biomarkers, Tumor / genetics*
  • Case-Control Studies
  • DNA, Intergenic*
  • Female
  • Genome, Mitochondrial*
  • Humans
  • Liposarcoma / genetics*
  • Male
  • Middle Aged
  • Polymorphism, Single Nucleotide*

Substances

  • Biomarkers, Tumor
  • DNA, Intergenic