Effective binding of a phosphatidylserine-targeting antibody to Ebola virus infected cells and purified virions

J Immunol Res. 2015:2015:347903. doi: 10.1155/2015/347903. Epub 2015 Mar 1.

Abstract

Ebola virus is responsible for causing severe hemorrhagic fevers, with case fatality rates of up to 90%. Currently, no antiviral or vaccine is licensed against Ebola virus. A phosphatidylserine-targeting antibody (PGN401, bavituximab) has previously been shown to have broad-spectrum antiviral activity. Here, we demonstrate that PGN401 specifically binds to Ebola virus and recognizes infected cells. Our study provides the first evidence of phosphatidylserine-targeting antibody reactivity against Ebola virus.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Viral / immunology*
  • Antibodies, Viral / metabolism
  • Cell Line
  • Cells, Cultured
  • Chlorocebus aethiops
  • Ebolavirus / immunology*
  • Ebolavirus / metabolism
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Hemorrhagic Fever, Ebola / immunology
  • Hemorrhagic Fever, Ebola / virology
  • Humans
  • Phosphatidylserines / immunology*
  • Phosphatidylserines / metabolism
  • Protein Binding / immunology
  • Vero Cells
  • Virion / immunology*
  • Virion / metabolism

Substances

  • Antibodies, Viral
  • Phosphatidylserines