Tetrahymena Expresses More than a Hundred Proteins with Lipid-binding MORN Motifs that can Differ in their Subcellular Localisations

J Eukaryot Microbiol. 2015 Sep-Oct;62(5):694-700. doi: 10.1111/jeu.12216. Epub 2015 Apr 25.

Abstract

Proteins with membrane occupation and recognition nexus (MORN) motifs are associated with cell fission in apicomplexan parasites, chloroplast division in Arabidopsis and the motility of sperm cells. We found that ciliates are among those that encode the largest variety of MORN proteins. Tetrahymena thermophila expresses 129 MORN protein-encoding genes, some of which are specifically up-regulated during conjugation. A lipid-binding assay underpins the assumption that the predominant function of MORN motifs themselves is to confer the ability of lipid binding. The localisation of four MORN candidate proteins with similar characteristics highlights the functional diversity of this group especially in ciliates.

Keywords: Ciliates; MORN; membrane targeting; membrane tethering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs*
  • Amino Acid Sequence
  • Lipid Metabolism
  • Membrane Proteins / chemistry*
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Protozoan Proteins / chemistry*
  • Protozoan Proteins / isolation & purification
  • Protozoan Proteins / metabolism*
  • Tetrahymena thermophila / chemistry*
  • Tetrahymena thermophila / genetics
  • Tetrahymena thermophila / metabolism*

Substances

  • Membrane Proteins
  • Protozoan Proteins

Associated data

  • GENBANK/FJ789658