Decreased ferroportin promotes myeloma cell growth and osteoclast differentiation

Cancer Res. 2015 Jun 1;75(11):2211-21. doi: 10.1158/0008-5472.CAN-14-3804. Epub 2015 Apr 8.

Abstract

Iron homeostasis is disrupted in multiple myeloma, a difficult-to-cure plasma cell malignancy with lytic bone lesions. Here, we systematically analyzed iron gene expression signature and demonstrated that mRNA expression of iron exporter ferroportin (FPN1) is significantly downregulated in myeloma cells and correlates negatively with clinic outcome. Restoring expression of FPN1 reduces intracellular liable iron pool, inhibits STAT3-MCL-1 signaling, and suppresses myeloma cells growth. Furthermore, we demonstrated that mRNA of FPN1 is also downregulated at the initial stages of osteoclast differentiation and suppresses myeloma cell-induced osteoclast differentiation through regulating iron regulator TFRC, NF-κB, and JNK pathways. Altogether, we demonstrated that downregulation of FPN1 plays critical roles in promoting myeloma cell growth and bone resorption in multiple myeloma.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / biosynthesis
  • Bone Resorption / genetics
  • Bone Resorption / pathology
  • Cation Transport Proteins / biosynthesis*
  • Cation Transport Proteins / genetics
  • Cell Differentiation
  • Cell Line, Tumor
  • Cell Proliferation / genetics*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Iron / metabolism*
  • Mice
  • Multiple Myeloma / genetics*
  • Multiple Myeloma / metabolism
  • Multiple Myeloma / pathology
  • NF-kappa B / biosynthesis
  • Osteoclasts / metabolism
  • Osteoclasts / pathology
  • Receptors, Transferrin / biosynthesis
  • STAT3 Transcription Factor / biosynthesis
  • Signal Transduction / genetics

Substances

  • Antigens, CD
  • CD71 antigen
  • Cation Transport Proteins
  • NF-kappa B
  • Receptors, Transferrin
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • metal transporting protein 1
  • Iron