A mitochondrion targeting fluorescent probe for imaging of intracellular superoxide radicals

Chem Commun (Camb). 2015 May 7;51(37):7931-4. doi: 10.1039/c5cc01075f.

Abstract

An amine-reactive fluorogenic molecule specifically turned on by superoxide radicals (O2˙(-)) was synthesized and coupled to a mitochondrial (MT) targeting peptide. The obtained probe showed superior uptake and MT targeting capabilities; and successfully detected the change in O2˙(-) levels in cells treated with chemical stimuli or single-walled carbon nanotubes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amines / chemical synthesis
  • Amines / chemistry*
  • Animals
  • Cell Line
  • Fluorescent Dyes / analysis
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism*
  • Macrophages / cytology
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice
  • Mitochondria / metabolism*
  • Molecular Structure
  • Nanotubes, Carbon / chemistry
  • Superoxides / analysis*
  • Superoxides / chemistry

Substances

  • Amines
  • Fluorescent Dyes
  • Nanotubes, Carbon
  • Superoxides