Plant-based production of two chimeric monoclonal IgG antibodies directed against immunodominant epitopes of Vibrio cholerae lipopolysaccharide

J Immunol Methods. 2015 Jul:422:111-7. doi: 10.1016/j.jim.2015.04.001. Epub 2015 Apr 9.

Abstract

We have produced and characterized two chimeric human IgG1 monoclonal antibodies that bind different immunodominant epitopes on Vibrio cholerae lipopolysaccharide (LPS). MAb 2D6 IgG1 recognizes Ogawa O-polysaccharide antigen, while mAb ZAC-3 IgG1 recognizes core/lipid A moiety of Ogawa and Inaba LPS. Both antibodies were expressed using a Nicotiana benthamiana-based rapid antibody-manufacturing platform (RAMP) and evaluated in vitro for activities associated with immunity to V. cholerae, including vibriocidal activity, bacterial agglutination and motility arrest.

Keywords: Antibodies; Cholera; Plant expression; Vaccine.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antibodies, Bacterial / biosynthesis
  • Antibodies, Bacterial / genetics
  • Antibodies, Bacterial / immunology*
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / genetics
  • Antibodies, Monoclonal / immunology*
  • Antigens, Bacterial / immunology
  • Chimera / immunology
  • Cloning, Molecular
  • Epitopes / immunology
  • Humans
  • Immunodominant Epitopes / immunology*
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / genetics
  • Immunoglobulin G / immunology*
  • Lipopolysaccharides / immunology
  • Nicotiana / genetics
  • O Antigens / immunology
  • Vibrio cholerae / immunology*

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Epitopes
  • Immunodominant Epitopes
  • Immunoglobulin G
  • Lipopolysaccharides
  • O Antigens