Performance of ARCHITECT HCV core antigen test with specimens from US plasma donors and injecting drug users

J Clin Virol. 2015 May:66:15-8. doi: 10.1016/j.jcv.2015.02.015. Epub 2015 Feb 24.

Abstract

Background: Hepatitis C virus (HCV) core antigen is a serological marker of current HCV infection.

Objectives: The aim of this study was mainly to evaluate the performance characteristics of the ARCHITECT HCV core antigen assay with specimens from US plasma donors and injecting drug users.

Study design: A total of 551 serum and plasma samples with known anti-HCV and HCV RNA status were tested for HCV core antigen using the Abbott ARCHITECT HCV core antigen test.

Results: HCV core antigen was detectable in 100% of US plasma donor samples collected during the pre-seroconversion phase of infection (anti-HCV negative/HCV RNA positive). Overall sensitivity of the HCV core antigen assay was 88.9-94.3% in samples collected after seroconversion. The correlation between HCV core antigen and HCV RNA titers was 0.959.

Conclusions: HCV core antigen testing may be reliably used to identify current HCV infection.

Keywords: HCV RNA; HCV core antigen; HCV screening.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Viral / blood*
  • Blood Donors*
  • Diagnostic Tests, Routine / methods*
  • Drug Users*
  • Hepatitis C / diagnosis*
  • Humans
  • Immunoassay / methods
  • Mass Screening / methods*
  • Plasma / virology*
  • Sensitivity and Specificity
  • Substance Abuse, Intravenous / complications*
  • United States
  • Viral Core Proteins / blood*

Substances

  • Antigens, Viral
  • Viral Core Proteins
  • nucleocapsid protein, Hepatitis C virus