Analysis of Il-10 gene sequence in patients with sinonasal polyposis

Int J Immunopathol Pharmacol. 2015 Sep;28(3):434-9. doi: 10.1177/0394632015573922. Epub 2015 Apr 8.

Abstract

Sinonasal polyposis (SNP) is a chronic inflammatory disease of nasal and paranasal cavities. Human leukocyte antigen-G molecules (HLA-G) are non-classic HLA-I molecules with anti-inflammatory and tolerogenic properties. HLA-G production is mainly induced by interleukin (IL)-10. IL-10 is an anti-inflammatory cytokine that inhibits the production of proinflammatory cytokines and induces HLA-class II down-modulation. Recent studies suggest that HLA-G could play a role in SNP pathogenesis; in SNP patients physiological levels of IL-10 (produced by activated peripheral blood CD14+ monocytes) are not able to induce production of HLA-G. Different mechanisms could justify these findings: genomic or amino-acidic sequence alterations in IL-10 lower IL-10 receptor expression, lower IL-10 receptor affinity, or alterations of the intracellular signal transmission. This study analyzes nucleotidic sequence of IL-10 gene in SNP patients. Sequencing of IL-10 gene shows that the lack of HLA-G production by peripheral blood CD14+ monocytes is not related to alterations in IL-10 gene nucleotidic sequence.

Keywords: HLA-G; interleukin (IL)-10 cytokine; nasal inflammatory disease; nasal polyposis; nucleotide sequence.

Publication types

  • Letter

MeSH terms

  • Adult
  • Cytokines / genetics
  • Female
  • HLA-G Antigens / genetics
  • Histocompatibility Antigens Class I / genetics
  • Humans
  • Interleukin-10 / genetics*
  • Lipopolysaccharide Receptors / genetics
  • Male
  • Monocytes / metabolism
  • Nasal Polyps / genetics*
  • Receptors, Interleukin-10 / genetics

Substances

  • Cytokines
  • HLA-G Antigens
  • Histocompatibility Antigens Class I
  • IL10 protein, human
  • Lipopolysaccharide Receptors
  • Receptors, Interleukin-10
  • Interleukin-10