Motor neuron transduction after intracisternal delivery of AAV9 in a cynomolgus macaque

Hum Gene Ther Methods. 2015 Apr;26(2):43-4. doi: 10.1089/hgtb.2015.041.

Abstract

The image shows a section of the lumbar spinal cord from a cynomolgus macaque that had received AAV9.CB.EGFP via the cisterna magna. Expression of GFP in multiple motor neurons is visible. Injection into the cerebrospinal fluid has been shown to be an effective route of vector administration for neuron transduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cisterna Magna / metabolism
  • Dependovirus / genetics*
  • Genetic Vectors*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Injections, Spinal
  • Lumbar Vertebrae / anatomy & histology
  • Macaca fascicularis
  • Motor Neurons / metabolism*
  • Promoter Regions, Genetic
  • Spinal Cord / anatomy & histology
  • Transduction, Genetic*

Substances

  • Green Fluorescent Proteins