Rapid Detection of Acquired and Inducible Clarithromycin Resistance in Mycobacterium abscessus Group by a Simple Real-Time PCR Assay

Robert F Luo; Cheyenne Curry; Nathan Taylor; Indre Budvytiene; Niaz Banaei

doi:10.1128/JCM.00132-15

Rapid Detection of Acquired and Inducible Clarithromycin Resistance in Mycobacterium abscessus Group by a Simple Real-Time PCR Assay

J Clin Microbiol. 2015 Jul;53(7):2337-9. doi: 10.1128/JCM.00132-15. Epub 2015 Apr 22.

Authors

Robert F Luo¹, Cheyenne Curry², Nathan Taylor², Indre Budvytiene², Niaz Banaei³

Affiliations

¹ Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
² Clinical Microbiology Laboratory, Stanford Health Care, Palo Alto, California, USA.
³ Department of Pathology, Stanford University School of Medicine, Stanford, California, USA Clinical Microbiology Laboratory, Stanford Health Care, Palo Alto, California, USA Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA [email protected].

Abstract

By targeting the erm(41) and rrl genes in the Mycobacterium abscessus group, a multiplex real-time PCR assay for clarithromycin resistance showed 95% (38/40) concordance with nucleic acid testing and 95% (37/39) concordance with phenotypic testing. This assay provides a simple and rapid alternative to extended incubation or erm(41) sequencing.

Publication types

Evaluation Study

MeSH terms

Anti-Bacterial Agents / pharmacology*
Clarithromycin / pharmacology*
Drug Resistance, Bacterial*
Gene Expression Regulation, Bacterial / drug effects
Genotype
Humans
Microbial Sensitivity Tests
Multiplex Polymerase Chain Reaction / methods*
Mycobacterium Infections, Nontuberculous / microbiology
Nontuberculous Mycobacteria / drug effects*
Nontuberculous Mycobacteria / genetics
Nontuberculous Mycobacteria / isolation & purification
Phenotype
Real-Time Polymerase Chain Reaction / methods*
Texas
Time Factors

Substances

Anti-Bacterial Agents
Clarithromycin