Abstract
Here, we report the evolution of an orthogonal amber suppressor pyrrolysyl-tRNA synthetase (PylRS)/tRNACUA(Pyl) pair that genetically encodes the post-translationally modified amino acid, ε-N-2-hydroxyisobutyryl-lysine (HibK), in bacteria and mammalian cells. HibK is a new type of histone mark that is widely distributed in histone proteins. The ability to site-specifically incorporate HibK into proteins provides a useful tool to probe the biological function of this newly identified post-translational modification.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acyl-tRNA Synthetases / genetics
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Amino Acyl-tRNA Synthetases / metabolism
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Animals
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression
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HEK293 Cells
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Histones / genetics*
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Histones / metabolism
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Humans
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Lysine / analogs & derivatives*
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Lysine / genetics*
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Models, Molecular
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Protein Processing, Post-Translational
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Histones
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Recombinant Proteins
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Amino Acyl-tRNA Synthetases
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Lysine