Molecular cloning and characterization of the complementary DNA coding for the B-chain of murine Clq

FEBS Lett. 1989 Nov 20;258(1):89-93. doi: 10.1016/0014-5793(89)81622-9.

Abstract

cDNA clones coding for the B-chain of murine Clq were isolated from a mouse macrophage library. The characterized clones include the total coding region plus a leader sequence. High homology was found with human Clq B-chain in the coding region (81%). Northern blot analysis of total RNA from different tissues of Balb/c mice showed one band of approximately 1.2 kb. The highest signal was found in RNA preparations of thioglycolate-activated peritoneal macrophages. The probe also hybridized with mRNA from spleen, thymus and heart. Extremely weak signals were found in liver, kidney, lung and intestine tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Cloning, Molecular
  • Complement C1q / analysis
  • Complement C1q / genetics*
  • DNA / analysis*
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Promoter Regions, Genetic
  • Protein Sorting Signals / analysis
  • RNA Probes
  • RNA, Messenger / analysis*
  • Sequence Homology, Nucleic Acid

Substances

  • Protein Sorting Signals
  • RNA Probes
  • RNA, Messenger
  • Complement C1q
  • DNA