Increasing Vero viable cell densities for yellow fever virus production in stirred-tank bioreactors using serum-free medium

Diogo A Mattos; Marlon V Silva; Luciane P Gaspar; Leda R Castilho

doi:10.1016/j.vaccine.2015.04.050

Increasing Vero viable cell densities for yellow fever virus production in stirred-tank bioreactors using serum-free medium

Vaccine. 2015 Aug 20;33(35):4288-91. doi: 10.1016/j.vaccine.2015.04.050. Epub 2015 Apr 27.

Authors

Diogo A Mattos¹, Marlon V Silva¹, Luciane P Gaspar², Leda R Castilho³

Affiliations

¹ Bio-Manguinhos-FIOCRUZ, Technological Development Vice-directory, Rio de Janeiro, RJ, Brazil.
² Bio-Manguinhos-FIOCRUZ, Technological Development Vice-directory, Rio de Janeiro, RJ, Brazil. Electronic address: [email protected].
³ Federal University of Rio de Janeiro (UFRJ), COPPE, Chemical Engineering Program, Rio de Janeiro, RJ, Brazil.

PMID: 25930117
DOI: 10.1016/j.vaccine.2015.04.050

Abstract

In this work, changes in Vero cell cultivation methods have been employed in order to improve cell growth conditions to obtain higher viable cell densities and to increase viral titers. The propagation of the 17DD yellow fever virus (YFV) in Vero cells grown on Cytodex I microcarriers was evaluated in 3-L bioreactor vessels. Prior to the current changes, Vero cells were repeatedly displaying insufficient microcarrier colonization. A modified cultivation process with four changes has resulted in higher cell densities and higher virus titers than previously observed for 17DD YFV.

Keywords: Bioreactors; Serum-free medium; Vero cells; Yellow fever virus production.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bioreactors*
Cell Count*
Cell Culture Techniques* / instrumentation
Cell Culture Techniques* / methods
Cell Survival
Chlorocebus aethiops
Culture Media, Serum-Free
Vero Cells
Viral Load
Virus Cultivation / methods*
Yellow fever virus / growth & development*

Substances

Culture Media, Serum-Free