Phosphorylation stoichiometry determination in plant photosynthetic membranes

Björn Ingelsson; Rikard Fristedt; Maria V Turkina

doi:10.1007/978-1-4939-2648-0_9

Phosphorylation stoichiometry determination in plant photosynthetic membranes

Methods Mol Biol. 2015:1306:121-34. doi: 10.1007/978-1-4939-2648-0_9.

Authors

Björn Ingelsson¹, Rikard Fristedt, Maria V Turkina

Affiliation

¹ Division of Cell Biology, Department of Clinical and Experimental Medicine, Linköping University, 581 85, Linköping, Sweden.

PMID: 25930698
DOI: 10.1007/978-1-4939-2648-0_9

Abstract

This chapter describes different strategies for the study of phosphorylation dynamics and stoichiometry in photosynthetic membranes. Detailed procedures for the detection, large-scale identification, and quantification of phosphorylated proteins optimized for plant thylakoid proteins are given.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism
Phosphoproteins / analysis*
Phosphorylation
Photosynthesis
Proteomics / methods*
Thylakoids / metabolism*

Substances

Arabidopsis Proteins
Phosphoproteins