Immunoblotting conditions for small peptides from streptococci

Lauren Davey; Scott A Halperin; Song F Lee

doi:10.1016/j.mimet.2015.04.015

Immunoblotting conditions for small peptides from streptococci

J Microbiol Methods. 2015 Jul:114:40-2. doi: 10.1016/j.mimet.2015.04.015. Epub 2015 May 1.

Authors

Lauren Davey¹, Scott A Halperin², Song F Lee³

Affiliations

¹ Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 1X5, Canada; Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada.
² Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 1X5, Canada; Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada; Department of Pediatrics, Faculty of Medicine, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada.
³ Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 1X5, Canada; Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada; Department of Pediatrics, Faculty of Medicine, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada; Department of Applied Oral Sciences, Faculty of Dentistry, Dalhousie University, Halifax, NS B3H 4R2, Canada. Electronic address: [email protected].

PMID: 25937086
DOI: 10.1016/j.mimet.2015.04.015

Abstract

Streptococci secrete small peptides with important biological functions. These peptides are not amenable to standard immunoblotting, and are often detected indirectly using activity assays, or by alternative approaches that may be expensive and laborious. Here we describe an immunoblotting method that enables reproducible detection of these small streptococcal peptides.

Keywords: Bacteriocins; Competence stimulating peptides; Immunoblotting; Streptococcal small peptides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Immunoblotting / methods*
Peptides / analysis*
Reproducibility of Results
Streptococcus / chemistry*

Substances

Peptides