Injectable Hyaluronan Hydrogels with Peptide-Binding Dendrimers Modulate the Controlled Release of BMP-2 and TGF-β1

Macromol Biosci. 2015 Aug;15(8):1035-44. doi: 10.1002/mabi.201500082. Epub 2015 May 5.

Abstract

BMP-2 and TGF-β1 released from injectable thermoresponsive hydrogels are studied in the presence and absence of branched macromolecules bearing BMP-2 or TGF-β1 affinity binding peptides. The synthesized branched macromolecules and the gelling compositions before and after loading with either BMP-2 or TGF-β1 are characterized physico-chemically and show a significantly lower amount of proteins released in the presence of the affinity binding peptide macromolecules. This study illustrates the potential of affinity binding peptide functionalized dendrimers to modulate the local delivery and availability of growth factors important for musculoskeletal regeneration therapies.

Keywords: dendrimers; drug delivery; hyaluronan; injectable hydrogels; nanotechnology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / chemistry
  • Bone Morphogenetic Protein 2 / chemistry*
  • Bone Morphogenetic Protein 2 / metabolism
  • Delayed-Action Preparations
  • Dendrimers / chemistry
  • Dendrimers / pharmacology
  • Drug Delivery Systems*
  • Humans
  • Hyaluronic Acid / chemistry*
  • Hyaluronic Acid / pharmacology
  • Hydrogels / chemistry
  • Hydrogels / pharmacology
  • Magnetic Resonance Spectroscopy
  • Nanotechnology
  • Peptides / chemistry
  • Peptides / pharmacology
  • Protein Binding
  • Rheology
  • Transforming Growth Factor beta1 / chemistry*
  • Transforming Growth Factor beta1 / metabolism

Substances

  • Amino Acids
  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • Delayed-Action Preparations
  • Dendrimers
  • Hydrogels
  • Peptides
  • Transforming Growth Factor beta1
  • Hyaluronic Acid