Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells

Sci Data. 2014 May 27:1:140008. doi: 10.1038/sdata.2014.8. eCollection 2014.

Abstract

The reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is lengthy and inefficient. The development of a reprogramming system that allows rapid and synchronous reprogramming to pluripotency is imperative for understanding the mechanism of iPSC formation and for future therapeutic applications. We have recently reported that a short expression in mouse primary B cells of the transcription factor C/EBPα before the induction of pluripotency factors increases the iPSC reprogramming efficiency >100-fold, involving 95% of the cells within a week. Here we present a dataset containing the time course of gene expression during this process as determined by microarray and RNA-seq techniques.

Publication types

  • Dataset

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / physiology
  • CCAAT-Enhancer-Binding Proteins / genetics
  • Cell Differentiation / genetics
  • Cellular Reprogramming / genetics*
  • Gene Expression
  • Gene Expression Profiling*
  • Induced Pluripotent Stem Cells* / cytology
  • Induced Pluripotent Stem Cells* / physiology
  • Mice
  • Microarray Analysis

Substances

  • CCAAT-Enhancer-Binding Proteins
  • CEBPA protein, mouse