Detection of Orthobunyavirus in mosquitoes collected in Argentina

Med Vet Entomol. 2015 Sep;29(3):338-43. doi: 10.1111/mve.12121. Epub 2015 May 20.

Abstract

Bunyamwera virus (BUNV) (Bunyaviridae, genus Orthobunyavirus, serogroup Bunyamwera) is considered an emerging pathogen for humans and animals in American countries. The CbaAr-426 strain of BUNV was recovered from mosquitoes Ochlerotatus albifasciatus (Diptera: Culicidae) collected in Córdoba province (Argentina), where serological studies detected high seroprevalences in humans and animals. Molecular detection of Orthobunyavirus was performed in mosquitoes collected in Córdoba province. Seventeen mosquito pools of Oc. albifasciatus, Ochlerotatus scapularis and Culex quinquefasciatus (Diptera: Culicidae) showed positive results; four of these positive pools, all of Oc. scapularis, were sequenced. All amplicons grouped with BUNV in the Bunyamwera serogroup. The findings highlight the circulation of BUNV in Córdoba province and represent the first report of BUNV-infected Oc. scapularis mosquitoes in Argentina.

Keywords: Argentina; Bunyamwera virus; Bunyaviridae; Córdoba; Orthobunyavirus; mosquitoes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Argentina
  • Culicidae / virology*
  • Female
  • Insect Vectors / virology*
  • Molecular Sequence Data
  • Nucleocapsid Proteins / genetics*
  • Orthobunyavirus / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA

Substances

  • Nucleocapsid Proteins

Associated data

  • GENBANK/KM234269
  • GENBANK/KM234270
  • GENBANK/KM234271
  • GENBANK/KM234272