Generation of a tamoxifen inducible Tnnt2MerCreMer knock-in mouse model for cardiac studies

Jianyun Yan; Nishat Sultana; Lu Zhang; David S Park; Akshay Shekhar; Jun Hu; Lei Bu; Chen-Leng Cai

doi:10.1002/dvg.22861

Generation of a tamoxifen inducible Tnnt2MerCreMer knock-in mouse model for cardiac studies

Genesis. 2015 Jun;53(6):377-86. doi: 10.1002/dvg.22861. Epub 2015 Jun 13.

Authors

Jianyun Yan¹, Nishat Sultana¹, Lu Zhang¹, David S Park², Akshay Shekhar², Jun Hu¹, Lei Bu², Chen-Leng Cai¹

Affiliations

¹ Department of Developmental and Regenerative Biology, The Black Family Stem Cell Institute, and the Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, New York.
² Leon H. Charney Division of Cardiology, New York University School of Medicine, 522 First Avenue, New York, New York.

Abstract

Tnnt2, encoding thin-filament sarcomeric protein cardiac troponin T, plays critical roles in heart development and function in mammals. To develop an inducible genetic deletion strategy in myocardial cells, we generated a new Tnnt2:MerCreMer (Tnnt2(MerCreMer/+)) knock-in mouse. Rosa26 reporter lines were used to examine the specificity and efficiency of the inducible Cre recombinase. We found that Cre was specifically and robustly expressed in the cardiomyocytes at embryonic and adult stages following tamoxifen induction. The knock-in allele on Tnnt2 locus does not impact cardiac function. These results suggest that this new Tnnt2(MerCreMer/+) mouse could be applied towards the temporal genetic deletion of genes of interests in cardiomyocytes with Cre-LoxP technology. The Tnnt2(MerCreMer/+) mouse model also provides a useful tool to trace myocardial lineage during development and repair after cardiac injury.

Keywords: MerCreMer; Tnnt2; cardiomyocyte; heart; tamoxifen.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Actins / metabolism
Animals
Antineoplastic Agents, Hormonal / pharmacology
Embryo, Mammalian / embryology
Embryo, Mammalian / metabolism
Female
Gene Expression Regulation / drug effects*
Heart / embryology
Heart / growth & development
Heart / physiology
Immunohistochemistry
Integrases / genetics
Integrases / metabolism
Male
Mice, Transgenic
Models, Animal
Muscle, Smooth / chemistry
Myocardium / cytology
Myocardium / metabolism*
Myocytes, Cardiac / metabolism
Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
RNA, Untranslated / genetics
Tamoxifen / pharmacology*
Time Factors
Troponin T / genetics*
Troponin T / metabolism
beta-Galactosidase / genetics
beta-Galactosidase / metabolism

Substances

Actins
Antineoplastic Agents, Hormonal
Gt(ROSA)26Sor non-coding RNA, mouse
Platelet Endothelial Cell Adhesion Molecule-1
RNA, Untranslated
Troponin T
Tamoxifen
Cre recombinase
Integrases
beta-Galactosidase

Abstract

Publication types

MeSH terms

Substances

Grants and funding